背景描述: WM266-4 是一种转移性人黑色素瘤细胞系,具有小扁平间充质形态。该细胞系来源于与细胞系 WM115、WM239A 和 WM165-1 相同的患者。WM115细胞系来源于原发肿瘤,WM266-4、WM165-1、WM239A来源于单个淋巴结转移瘤。受试者(55 岁女性)显示 VGP 伴 Clark III 级肿瘤,厚度为 2.24mm。
培养建议
生长形态: 小扁平间充质
生长特性: 贴壁
培养条件: DMEM+10%FBS+1%P/S
冻存条件: 90%FBS+10%DMSO
血清使用等级: 优级
传代方式: 胰酶消化
倍增时间: 30h
传代比例: 胰酶消化
换液频率: 每周2~3次
生物安全等级: 1
培养注意事项:
其他信息
基因表达: WM266-4 cell line is wild type for N-RAS, c-KIT, and CDK4 genes.
致瘤性: WM266-4 cells produce xenograft tumors when injected into immunocompromised mice.
突变信息: This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion.
人黑色素瘤细胞
Catalog NoCL0718
Product information
基因表达: WM266-4 cell line is wild type for N-RAS, c-KIT, and CDK4 genes.
致瘤性: WM266-4 cells produce xenograft tumors when injected into immunocompromised mice.
突变信息: This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion.