小鼠结缔组织L细胞株929克隆

> > >

小鼠结缔组织L细胞株929克隆(L929)

￥800.00元

细胞货号： CL0339

细胞种属： 小鼠细胞系

细胞别名： NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); Clone 929; Earles's cells; Earle's L cells

发货规格： 1×10⁶

供体信息： 100天雄性C3H/An小鼠

肿瘤类型：

组织来源： 正常皮下结缔组织

在线咨询

背景信息

种属：小鼠细胞系
组织来源：正常皮下结缔组织
肿瘤类型：
供体信息： 100天雄性C3H/An小鼠
背景描述： L-929 细胞是一种成纤维细胞样细胞系，来源于 100 日龄雄性 C3H/An 小鼠的皮下结缔组织。该细胞系成立于 1940 年代，由于其稳健性、易于培养和应用多功能性，已成为各种生物和医学研究领域的关键。L-929 细胞的特征在于其纺锤形、成纤维细胞形态和贴壁生长。它们广泛用于细胞毒性测定，并作为评估材料生物相容性和各种物质毒性作用的标准模型，这在生物材料和组织工程领域尤为重要。

培养建议

生长形态：成纤维细胞样
生长特性：贴壁
培养条件： RPMI-1640+10%FBS+1%P/S
冻存条件： 90%FBS+10%DMSO
血清使用等级：标准
传代方式：胰酶消化
倍增时间： 24~28h
传代比例：胰酶消化
换液频率：每周2~3次
生物安全等级： 1
培养注意事项：

其他信息

致瘤性： Yes, in immunosuppressed mice
抗原表达： H-2k
病毒： Ectromelia virus (mousepox): negative
病毒易感性： poliovirus 1, 2, 3, coxsackievirus B5, polyomavirus
逆转录酶：阳性
核型： modal chromosome number = 66; range = 65 to 68. There were approximately 20 to 30 marker chromosomes present in each metaphase spread. A high percentage of those markers were common to most analyzed cells. A long metacentric chromosome with secondary constriction was noted in 77/100 cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

小鼠结缔组织L细胞株929克隆

Catalog No CL0339

Product information

致瘤性： Yes, in immunosuppressed mice
抗原表达： H-2k
病毒： Ectromelia virus (mousepox): negative
病毒易感性： poliovirus 1, 2, 3, coxsackievirus B5, polyomavirus
逆转录酶：阳性
核型： modal chromosome number = 66; range = 65 to 68. There were approximately 20 to 30 marker chromosomes present in each metaphase spread. A high percentage of those markers were common to most analyzed cells. A long metacentric chromosome with secondary constriction was noted in 77/100 cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

Hunan UPT Biotechnology Co.,Ltd

Website：www.uptbio.com Servive hotline :4006916686

E-mail：service@uptbio.com

Address：

Room 402, Building 13, Xinggong International Industrial Park, 100 Guyuan Road, Yuelu District, Changsha City, Hunan Province, China.