人皮肤恶性黑色素瘤细胞

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人皮肤恶性黑色素瘤细胞(SK-MEL-28)

￥800.00元

细胞货号： CL0284

细胞种属： 人源细胞系

细胞别名： SK-Mel-28; SK.MEL.28; SK-MEL 28; SK MEL-28; SK MEL 28; SK Mel 28; SKMel-28; SKMEL-28; SK-MEL28; SK-Mel28; SK Mel28; SKMEL28; SKMel28; SKmel28; SKML-28; SK28; AU-Mel; P-36; P36

发货规格： 1×10⁶

供体信息： 51岁男性

肿瘤类型： 皮肤黑色素瘤

组织来源： 皮肤

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背景信息

种属：人源细胞系
组织来源：皮肤
肿瘤类型：皮肤黑色素瘤
供体信息： 51岁男性
背景描述：该细胞系是由 T.Takahashi 及其同事从一名种族不明的 51 岁男性的腋窝淋巴结中建立的，他们在一系列黑色素瘤系中分离出该细胞系。

培养建议

生长形态：多角形
生长特性：贴壁
培养条件： RPMI-1640+10%FBS+1%P/S
冻存条件： 90%FBS+10%DMSO
血清使用等级：标准
传代方式：胰酶消化
倍增时间： 27h
传代比例：胰酶消化
换液频率：每周2~3次
生物安全等级： 1
培养注意事项：

其他信息

蛋白表达： p53 positive
同工酶： PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B
致瘤性： Yes, in nude mice. Forms malignant melanoma (large round cell type)
抗原表达： Blood Type A; Rh+; HLA A11, A26, B40, DRw4
突变信息： BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot), N-Ras wt
核型： modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell.

人皮肤恶性黑色素瘤细胞

Catalog No CL0284

Product information

蛋白表达： p53 positive
同工酶： PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B
致瘤性： Yes, in nude mice. Forms malignant melanoma (large round cell type)
抗原表达： Blood Type A; Rh+; HLA A11, A26, B40, DRw4
突变信息： BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot), N-Ras wt
核型： modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell.

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