背景描述: 该系由A.F. Gazdar,H.K. Oie,J.D. Minna及其同事于1981年从患者开始化疗前获得的肿瘤组织中得出。超微结构研究表明,存在 Clara 细胞特征的细胞质结构。
培养建议
生长形态: 上皮细胞样
生长特性: 贴壁
培养条件: RPMI-1640+10%FBS+1%P/S
冻存条件: 90%FBS+10%DMSO
血清使用等级: 标准
传代方式: 胰酶消化
倍增时间: 38~60h
传代比例: 胰酶消化
换液频率: 每周2~3次
生物安全等级: 1
培养注意事项:
其他信息
基因表达: lung surfactant associated protein A (SP-A); protein and RNA of SP-A, the major lung surfactant associated protein
致瘤性: 裸鼠
核型: modal numbr = 57; range = 53 to 65.This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells.
人非小细胞肺癌细胞
Catalog NoCL0236
Product information
基因表达: lung surfactant associated protein A (SP-A); protein and RNA of SP-A, the major lung surfactant associated protein
致瘤性: 裸鼠
核型: modal numbr = 57; range = 53 to 65.This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells.