DNA Ligase IV Polyclonal Antibody

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商品货号： PLA015667

适应性： 人,大鼠,小鼠,

￥600元

规格：

40μL 100μL 200μL

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MSDS

说明书

商品描述

基因名称： LIG4
蛋白名称： DNA ligase 4
Human_gene_id： 3981
Human_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3981
Human_swiss_prot_no： P49917
Human_swiss_link： http://www.uniprot.org/uniprotkb/P49917/entry
Mouse_swiss_prot_no： Q8BTF7
Mouse_swiss_link： http://www.uniprot.org/uniprot/Q8BTF7
特异性： DNA Ligase IV Polyclonal Antibody detects endogenous levels of DNA Ligase IV protein.
组成： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源： Polyclonal, Rabbit,IgG
稀释： WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
纯化工艺： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
浓度： 1 mg/ml
储存： -15°C to -25°C/1 year(Do not lower than -25°C)
说明书： 1
Msds： MSDS_Antibody.pdf
其他名称： LIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
实测条带： 103kD
信号通路： Non-homologous end-joining;
功能： catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG4 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID) [MIM:602450]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Individuals affected by RS-SCID show defects in the DNA repair machinery necessary for coding joint formation and the completion of V(D)J recombination. A subset of cells from such patients show increased radiosensitivity.,disease:Defects in LIG4 are the cause of LIG4 syndrome [MIM:606593]. This disease is characterized by immunodeficiency and developmental and growth delay. Patients display unusual facial features, microcephaly, growth and/or developmental delay, pancytopenia, and various skin abnormalities.,function:Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.,online information:DNA ligase entry,online information:LIG4 mutation db,similarity:Belongs to the ATP-dependent DNA ligase family.,similarity:Contains 2 BRCT domains.,subunit:Binds to XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 heteromer associates in a DNA-dependent manner with the DNA-dependent protein kinase complex DNA-PK, formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC.,tissue specificity:Testis, thymus, prostate and heart.,
相关产品： RS0001,RS0002,YM3028,YM3029
细胞定位： Nucleus .
组织表达： Testis, thymus, prostate and heart.
科研货号： PLA015667

DNA Ligase IV Polyclonal Antibody

Catalog No PLA015667

Product information

基因名称： LIG4
蛋白名称： DNA ligase 4
Human_gene_id： 3981
Human_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3981
Human_swiss_prot_no： P49917
Human_swiss_link： http://www.uniprot.org/uniprotkb/P49917/entry
Mouse_swiss_prot_no： Q8BTF7
Mouse_swiss_link： http://www.uniprot.org/uniprot/Q8BTF7
特异性： DNA Ligase IV Polyclonal Antibody detects endogenous levels of DNA Ligase IV protein.
组成： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源： Polyclonal, Rabbit,IgG
稀释： WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
纯化工艺： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
浓度： 1 mg/ml
储存： -15°C to -25°C/1 year(Do not lower than -25°C)
说明书： 1
Msds： MSDS_Antibody.pdf
其他名称： LIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
实测条带： 103kD
信号通路： Non-homologous end-joining;
功能： catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG4 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID) [MIM:602450]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Individuals affected by RS-SCID show defects in the DNA repair machinery necessary for coding joint formation and the completion of V(D)J recombination. A subset of cells from such patients show increased radiosensitivity.,disease:Defects in LIG4 are the cause of LIG4 syndrome [MIM:606593]. This disease is characterized by immunodeficiency and developmental and growth delay. Patients display unusual facial features, microcephaly, growth and/or developmental delay, pancytopenia, and various skin abnormalities.,function:Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.,online information:DNA ligase entry,online information:LIG4 mutation db,similarity:Belongs to the ATP-dependent DNA ligase family.,similarity:Contains 2 BRCT domains.,subunit:Binds to XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 heteromer associates in a DNA-dependent manner with the DNA-dependent protein kinase complex DNA-PK, formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC.,tissue specificity:Testis, thymus, prostate and heart.,
相关产品： RS0001,RS0002,YM3028,YM3029
细胞定位： Nucleus .
组织表达： Testis, thymus, prostate and heart.
科研货号： PLA015667

Hunan UPT Biotechnology Co.,Ltd

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