Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody

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商品货号： PLA004288

适应性： 人,小鼠,大鼠

￥600元

规格：

40μL 100μL 200μL

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MSDS

说明书

商品描述

基因名称： CASP3
蛋白名称： Caspase3
Human_gene_id： 836
Human_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=836
Human_swiss_prot_no： P42574
Human_swiss_link： http://www.uniprot.org/uniprotkb/P42574/entry
Mouse_gene_id： 12367
Mouse_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12367
Mouse_swiss_prot_no： P70677
Mouse_swiss_link： http://www.uniprot.org/uniprot/P70677
Rat_gene_id： 25402
Rat_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25402
Rat_swiss_prot_no： P55213
Rat_swiss_link： http://www.uniprot.org/uniprot/P55213
特异性： Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-3 p17 protein resulting from cleavage adjacent to D175.
组成： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源： Polyclonal, Rabbit,IgG
稀释： WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300
纯化工艺： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
浓度： 1 mg/ml
储存： -15°C to -25°C/1 year(Do not lower than -25°C)
说明书： 1
Msds： MSDS_Antibody.pdf
其他名称： CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
实测条带： 17 34kD
信号通路： MAPK_ERK_Growth;MAPK_G_Protein;p53;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Natural killer cell mediated cytotoxicity;Alzheimer's disease;Parkinson's disease;Amyotrophic lateral sclerosis (ALS);Huntington's disease;Epithelial cell signaling in Helicobacter pylori infection;Pathways in cancer;Colorectal cancer;Viral myocarditis;
功能： catalytic activity:Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.,enzyme regulation:Inhibited by isatin sulfonamides.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.,PTM:Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.,PTM:S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.,tissue specificity:Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.,
相关产品： RS0001,RS0002,YM3028,YM3029
细胞定位： Cytoplasm.
组织表达： Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
tag： hot
科研货号： PLA004288

Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody

Catalog No PLA004288

Product information

基因名称： CASP3
蛋白名称： Caspase3
Human_gene_id： 836
Human_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=836
Human_swiss_prot_no： P42574
Human_swiss_link： http://www.uniprot.org/uniprotkb/P42574/entry
Mouse_gene_id： 12367
Mouse_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12367
Mouse_swiss_prot_no： P70677
Mouse_swiss_link： http://www.uniprot.org/uniprot/P70677
Rat_gene_id： 25402
Rat_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25402
Rat_swiss_prot_no： P55213
Rat_swiss_link： http://www.uniprot.org/uniprot/P55213
特异性： Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-3 p17 protein resulting from cleavage adjacent to D175.
组成： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源： Polyclonal, Rabbit,IgG
稀释： WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300
纯化工艺： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
浓度： 1 mg/ml
储存： -15°C to -25°C/1 year(Do not lower than -25°C)
说明书： 1
Msds： MSDS_Antibody.pdf
其他名称： CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
实测条带： 17 34kD
信号通路： MAPK_ERK_Growth;MAPK_G_Protein;p53;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Natural killer cell mediated cytotoxicity;Alzheimer's disease;Parkinson's disease;Amyotrophic lateral sclerosis (ALS);Huntington's disease;Epithelial cell signaling in Helicobacter pylori infection;Pathways in cancer;Colorectal cancer;Viral myocarditis;
功能： catalytic activity:Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.,enzyme regulation:Inhibited by isatin sulfonamides.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.,PTM:Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.,PTM:S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.,tissue specificity:Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.,
相关产品： RS0001,RS0002,YM3028,YM3029
细胞定位： Cytoplasm.
组织表达： Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
tag： hot
科研货号： PLA004288

Hunan UPT Biotechnology Co.,Ltd

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